Composite

Part:BBa_K4015009:Design

Designed by: Andrew Peng Yi   Group: iGEM21_KEYSTONE   (2021-10-19)


CotA-HlyA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 286


Design Notes

Both of them have a secretory function, which leads to the fact that connecting them in one piece hinders the function of both causing the failure to secrete the product.


Source

The parts are assembled by Gibson assembly, and is ligated into pET 23a.

References